A renaissance for the pioneering 16S rRNA gene
نویسندگان
چکیده
منابع مشابه
DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal primers is used for the amplification of the conserved region of the 16S rRNA gene, in common bacterial pathogens. Therefore, using the universal PCR method, these bacteria are detectable on...
متن کاملMolecular Identification of the Persian Gulf Sea Hare (Aplysia sp.) Based on 16s rRNA Gene Sequence
Background: Sea hares of the Aplysia genus are among the mollusks of interest for various researchers to study their phylogeny, bioactive compounds and the nervous system. These mollusks are herbivorous and produce chemical compounds (ink) to defend themselves. The present study provided molecular identification of the Persian Gulf (Bushehr city) sea hare using 16s rRNA gene sequence. Materials...
متن کاملTranscriptional analysis of the 16s rRNA gene in Rickettsia prowazekii.
The control of rRNA synthesis in the etiological agent of epidemic typhus, Rickettsia prowazekii, a slowly growing obligate intracytoplasmic bacterium, was investigated. Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only a single copy, was controlled by a single promoter region, and the site for the initiation of transcription (base A) was found 117 bp upstream of the ...
متن کاملIntravaginal microbial flora by the 16S rRNA gene sequencing.
OBJECTIVE Conventional diagnosis of bacterial vaginosis contains some controversial points. To understand accurately the relationship between clinical stages and the microbiotas, the intravaginal microbial flora was analyzed by the clone library method. STUDY DESIGN Vaginal fluid samples from 31 patients were examined. Lactobacillary grade, Nugent score, culture-based method, and clone librar...
متن کاملGenetic variations of avian Pasteurella multocida as demonstrated by 16S-23S rRNA gene sequences comparison
Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates...
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ژورنال
عنوان ژورنال: Current Opinion in Microbiology
سال: 2008
ISSN: 1369-5274
DOI: 10.1016/j.mib.2008.09.011